Liver Transcriptomes in C57BL/6 mice. Liver Transcriptomes in C57BL/6 mice

Purpose: a transcriptome analysis was used to ascertain whether lipid metabolism disorders were induced by high-fat diet (HFD), and comparisons were made between control, HFD and DfCS-Ib(treatment) groups. Methods: liver mRNA profiles of C57BL/6 mice were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: HFD could change the expression of some genes and disturb normal metabolisms. However, there were fewer changes for genes of DfCS-Ib group mice. According to the KEGG Pathway Database and GO analysis, genes involving in lipid biosynthesis, degradation, and transportation were changed by HFD. Conclusions: Our study indicated that DfCS-Ib alleviated lipid disorder of HFD-fed mice in liver by normalizing the expressions of related genes. Overall design: total liver mRNA profiles of C57BL/6 treated with sulfated oligosaccharide were generated by deep sequencing