Transcriptomics analysis of gene expression in wildtype and NSUN2 knockdown T24 cell
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https://www.ncbi.nlm.nih.gov/sra/SRP212699
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Total RNA was isolated using TRIzol® Reagent (Ambion). The RNA concentration and quality were determined with NanoDrop, Qubit and agarose gel analysis. The Dynabeads® mRNA Purification Kit (Ambion) was used to enrich the mRNAs, which were used as templates for RNA-Seq library construction. RNA-Seq libraries was directly constructed by using KAPA mRNA Stranded mRNA-Seq Kit (KAPA) according to the manufacturer's instructions. The libraries were sequenced on the Illumina HiSeq X-Ten platform at Novogene (Tianjin, CA) with paired-end 150 bp read length. The different genes expression between the control and NSUN2 knockdown cell were detected. Overall design: Examination of gene expressive levels in wildtype and NSUN2 knockdown T24 cell
创建时间：
2020-04-24
