File S1 - The Design and In Vivo Evaluation of Engineered I-OnuI-Based Enzymes for HEG Gene Drive

Combined supplementary information file containing Figures S1–S6 and Table S1. Figure S1, Schematic of an approach to isolating the AGAP004734 gene targeting nucleases using yeast surface display technology. Figure S2, Homing assay. Figure S3, Schematic of PCR primer locations. Figure S4, Sequence alignment of the wild type I-OnuI, two engineered I-OnuI variants cleaving the AGAP004734 gene target (H4734A and H4734B), and their parental enzyme (parental I-OnuI). Figure S5, Positions of substituted residues in the H4734A (a) and H4734B (b) endonucleases. Figure S6, Repair Lesions. Table S1, PCR analysis of repair products of I-OnuI derivatives. (PDF)