Detection of pap31 in <i>L. longipalpis</i> sand flies from day (D) 1 to D9 post feeding on blood infected with <i>B. bacilliformis</i> by qPCR and LAMP.

A total of 12 sand flies at D5 includes 4-individual sand flies plus 2 pools (4 SF/pool) (No. of samples = 6). A total of 16 sand flies at D7 includes 4-individual sand flies plus 3 pools (4 SF/pool) (No. of samples = 7). A total of 20 sand flies at D9 includes 4-individual plus 4 pools (4 SF/pool) (No. of samples = 8). *LAMP was considered positive when both duplicates were positive. #Proportion of samples positive by qPCR from all samples tested on each day. Detection of pap31 in L. longipalpis sand flies from day (D) 1 to D9 post feeding on blood infected with B. bacilliformis by qPCR and LAMP.