Data from: Reduced skin bacterial diversity correlates with increased pathogen infection intensity in an endangered amphibian host

2018 Ellison Molecular Ecology Rana sierrae skin microbiome Illumina sequencesMetabarcoding sequences generated using an Illumina MiSeq. Paired-end reads have been joined and demultiplexed using QIIME (this file is equivalent to the "seqs.fna" file generated by the QIIME script "multiple_split_libraries_fastq.py").2018_Ellison_Rsierrae_microbiome_Illumina_sequences.fna2018 Ellison Molecular Ecology Rana sierrae Bd resultsBd results (qPCR) and associated metadata for all Rana sierrae included in the study.2018_Ellison_Rsierrae_Bd_results.csv2018 Ellison Molecular Ecology Rana sierrae microbiome QIIME mapping fileMapping file containing sample metadata for all microbiome samples included in the study. Used to analyze microbial communities in QIIME.2018_Ellison_Rsierrae_microbiome_mapping_file.csv2018 Ellison Molecular Ecology Rana sierrae CFS inhibition trial resultsResults for the cell-free supernatant inhibition trials conducted using bacteria isolated from the skin of Rana sierrae.2018_Ellison_Rsierrae_CFS_inhibition_results.csv2018 Ellison Molecular Ecology Rana sierrae CFS inhibition sequencesFASTA file containing DNA sequences for the bacterial isolates included in the cell-free supernatant inhibition trials.2018_Ellison_Rsierrae_CFS_inhibition_sequences.txt本数据集包含2018年Ellison团队发表于《分子生态学（Molecular Ecology）》的内华达林蛙（Rana sierrae）相关研究的五组数据，具体如下：1. 内华达林蛙皮肤微生物组Illumina测序数据：采用Illumina MiSeq（Illumina MiSeq）平台生成的宏条形码测序序列，已通过QIIME（QIIME）完成配对末端读段的拼接与解复用，该文件等价于QIIME脚本"multiple_split_libraries_fastq.py"生成的"seqs.fna"文件，对应数据文件为`2018_Ellison_Rsierrae_microbiome_Illumina_sequences.fna`。2. 内华达林蛙蛙壶菌（Bd）检测结果：包含本研究中所有内华达林蛙样本的Bd实时荧光定量PCR（qPCR）检测结果及配套元数据，对应数据文件为`2018_Ellison_Rsierrae_Bd_results.csv`。3. 内华达林蛙微生物组QIIME映射文件：包含本研究中所有微生物组样本的样本元数据，用于QIIME平台的微生物群落分析，对应数据文件为`2018_Ellison_Rsierrae_microbiome_mapping_file.csv`。4. 内华达林蛙无细胞上清液（CFS）抑菌试验结果：包含从内华达林蛙皮肤分离得到的细菌所开展的CFS抑菌试验结果，对应数据文件为`2018_Ellison_Rsierrae_CFS_inhibition_results.csv`。5. 内华达林蛙CFS抑菌试验菌株测序序列：包含本CFS抑菌试验中所用分离菌株的DNA序列，存储为FASTA（FASTA）格式文件，对应数据文件为`2018_Ellison_Rsierrae_CFS_inhibition_sequences.txt`。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于濒危两栖动物Sierra Nevada黄腿蛙（Rana sierrae）皮肤微生物组与真菌病原体Batrachochytrium dendrobatidis（Bd）感染的关联研究。数据包括16S rRNA代谢条形码序列、Bd感染结果、微生物组映射文件和细菌抑制实验数据，揭示了高Bd感染强度下皮肤细菌多样性显著降低，且细菌代谢物具有抑制Bd生长的潜力，为开发抗Bd益生菌治疗提供了科学基础。