RNA sequencing of nuclease dead PET127 conditions

PET127, is thought to be a mitochondrial RNA exonuclease and loss
of PET127 is thought to cause accumulation of untrimmed
RNA in the mitochondria. Here we show RNA sequencing of
Saccharomyces cerevisiae in four PET127 conditions:
PET127 wild-type, pet127∆, pet127-nd (nuclease
dead)/pet127∆, and pet127-nd/PET127. The resulting
sequences show that regions of mitochondrial RNA accumulate in
the pet127∆ background, and the RNA sequencing
profile of PET127 containing disruptive mutations in the
conserved exonuclease active site mimics that
of pet127∆.