DNA methylation patterns in monocytes associate and evolve with prognosis in undifferentiated arthritis [MACs]

Ontogenic origin of monocytic cells in the synovial fluid is currently under discussion. Also, the polarization of these cells have been described to a play a role in the pathogenic joints of arthritis patients. To answer these questions, we analyzed data from blood and SF monocytes and compared them with those of in vitro-generated monocyte-derived macrophages. All conditions were compared, and differences were characterized, considering experimental condition (MO, M-CSF or GM-CSF), compartment (blood or SF) and patient prognosis (good or poor). Peripheral blood monocytes were purified using CD14 magnetic-activated cell sorting (MACS © MicroBeads, Miltenyi Biotec) and cultured in RPMI 1640 in the presence of macrophage colony-stimulating factor (M-CSF) or macrophage-granulocyte colony-stimulating factor (GM-CSF) for 5 days.