Transcriptomic Profiling across Human Serotonin Neuron Differentiation via the FEV Reporter System

This study has successfully established a human FEV reporter system using CRISPR/Cas9 gene-editing technology. We have investigated the precise timing of FEV expression in human SNs. When compared to the previously established TPH2-reporter system, the FEV-reporter system has demonstrated the capacity to yield a greater number of highly-purified SNs, which can be utilized for in-depth exploration of the transcriptomic profiles of SNs during the differentiation process. Moreover, this expanded yield of purified SNs holds promise for facilitating high-throughput drug screening. Overall design: Comparative gene expression profiling of RNA-seq data from cells differentiated from hESCs to human serotonin neurons at three time points (Day0 (hESCs), Day21, Day 28, Day42)