Bidirectional dysregulation of synaptic glutamate signaling after transient metabolic failure

Ischemia leads to a severe dysregulation of glutamate homeostasis and excitotoxic cell damage in the brain. Shorter episodes of energy depletion, for instance during peri-infarct depolarizations, can also acutely perturb glutamate signaling. It is less clear if such episodes of metabolic failure also have persistent effects on glutamate signaling and how the relevant mechanisms such as glutamate release and uptake are differentially affected. We modeled acute and transient metabolic failure by using a chemical ischemia protocol and analyzed its effect on glutamatergic synaptic transmission and extracellular glutamate signals by electrophysiology and multiphoton imaging, respectively, in the mouse hippocampus. Our experiments uncover a duration-dependent bidirectional dysregulation of glutamate signaling. Whereas short chemical ischemia induces a lasting potentiation of presynaptic glutamate release and synaptic transmission, longer episodes result in a persistent postsynaptic failure of..., See manuscript by Passlick et al. for a complete description of data acquisition and analysis., , # Bidirectional dysregulation of synaptic glutamate signaling after transient metabolic failure

[https://doi.org/10.5061/dryad.qjq2bvqr1](https://doi.org/10.5061/dryad.qjq2bvqr1)

## Description of the data and file structure from

The data set contains the main experimental results from the article (primary work, see above), also indicated below. Please see there for a detailed description of data acquisition, analysis, interpretation and discussion. A previous version of the manuscript is also available on bioRxiv ([https://www.biorxiv.org/content/10.1101/2024.04.11.588988v2](https://www.biorxiv.org/content/10.1101/2024.04.11.588988v2)).

**Bidirectional dysregulation of synaptic glutamate signaling after transient metabolic failure**

Stefan Passlick 1, Ghanim Ullah 2, Christian Henneberger 1, 3, *

1 Institute of Cellular Neurosciences, Medical Faculty, University of Bonn, Bonn, Germany, 2 Department of Physics, University of South Florida, Tampa, FL 33620, USA, 3 German Center fo...