Transcriptome sequencing of b1 treated HSC-T6 cells and STAT3 knockdown HSC-T6 cells

transcriptomic sequencing of HSC-T6 cells was performed to investigate the molecular mechanisms of how Saikosaponin b1 (Ssb1) inhibited the activation of HSCs by targeting STAT3. Differentially expressed genes (DEGs) regulated by Ssb1 and STAT3 were collected and analyzed with a Venn diagram. DEGs in TGF-beta1 vs TGF-beta1 + Ssb1 group and TGF-beta1 vs TGF-beta1 + shSTAT3 group were overlapped to recognize genes regulated by Ssb1 depending on STAT3. The results showed that 110 genes were upregulated and 179 genes were downregulated both in Ssb1-treated aHSCs and TGF-beta1-challenged STAT3-KD HSC-T6 cells.