Toxicity of LCDD monoclonal Ig free light chains on renal glomeruli

We analyzed the transcriptome (RNA-seq) of glomeruli in a mouse model of light chain deposition disease (LCDD) as compared to control mice (WT and DH-LMP2A, the latter producing no complete immunoglobulins, only free Ig light chains). Kidney lesions in LCDD are due to the deposition of an abnormal monoclonal free Ig light chain and comprise progressive thickening of basement membranes (tubular and glomerular) and nodular glomerulosclerosis resembling the lesions observed in diabetic nephropathy. The aim of the present study is to analyse the transcriptomic changes at early steps of glomerulosclerosis. Glomeruli were purified using magnetic beads as described in Takemoto et al. (2002). Each sample is the pool of 3 different mice except for WT1, WT2 and WT3, which are only one mouse each. Glomeruli are from 6-month-old mice, corresponding to the early steps of glomerulosclerosis.