nano16s demo datasets: Oxford Nanopore full-length 16S rRNA amplicon sequencing runs (Flongle, MinION, PromethION)

Five Oxford Nanopore sequencing runs of full-length 16S rRNA gene amplicons,provided as demonstration data for the nano16s analysis pipeline. The set spans three flow cell types, so it exercises the pipeline across arealistic range of run sizes and read depths: Flongle_Demo01 24 barcodes, ~400 MB (flow cell ASX982, 2024-04-17) Flongle_Demo02 16 barcodes, ~970 MB (flow cell ARA558, 2023-11-30) Flongle_Demo03 24 barcodes, ~680 MB (flow cell AYY777, 2025-03-25) MinION_Demo01 24 barcodes, ~4.3 GB (flow cell FBA90627, 2025-05-27) PromethION_Demo01 24 barcodes, ~4.9 GB (flow cell PBK40769, 2026-03-12) Each archive contains a fastq_pass/ directory with one barcode* subdirectoryper sample, as written by MinKNOW, together with the original ONT run report.Reads are basecalled and demultiplexed; fastq_fail and unclassified reads arenot included. One barcode directory corresponds to one physical sample, andbarcode numbering is not contiguous, so barcode number does not equal samplenumber. To analyse a dataset, point the pipeline at the fastq_pass directory: nano16s -d Flongle_Demo01/fastq_pass -o results Flongle_Demo01 is the smallest and is recommended for a first run. Reference figures from Flongle_Demo01: 200,318 reads across 24 barcodes;99.0% survived length and quality filtering (1,000-2,000 bp, Q>=10); medianread quality rose from 14.9 to 17.5; whole run 45 minutes on 19 cores.