AMH protects the ovary from doxorubicin by regulating cell fate and the response to DNA damage. AMH protects the ovary from doxorubicin by regulating cell fate and the response to DNA damage

we aim to explore the single-cell mechanisms behind ovarian damage in prepubertal mice following doxorubicin (DOX) treatment, and how Anti-Müllerian hormone (AMH) prevents chemotherapy-induced pathological damage, using single-cell RNA sequencing as well as quantitative and morphometric histological evaluation. Overall design: we treated mice (n=5) at the prepubertal age (postnatal day 25) with two weekly doses of DOX (3mg/kg). Additionally, we co-treated another group of mice with recombinant human AMH (rhAMH) (0.75mg/kg), the dose that was proven previously to protect the ovarian reserve to assess the protective effects of AMH. For comparison, we also included a group that received only rhAMH treatment. Ovaries were collected 4 hours after the second dose of DOX.