Data for: Why the lower reported prevalence of asthma in patients diagnosed with COVID-19 validates repurposing EDTA solutions to prevent and manage treat COVID-19 disease.

The NCBI pBlast tool was used to test the hypothesis that SARS-CoV-2 contains a calcium-dependent fusion domain(s) similar to those that were recently discovered in both Rubella and SARS-CoV-1. An Amino Acids comparison of the two relevant amino acid regions in S protein of SARS-CoV-1 representing fusion loop 1 (FL1 = Amino Acids 798- 819) and fusion loop 2 (FL2 = Amino Acids 835 -855) was conducted using the Protein Blast program from the National Center for Biotechnology Information. Specifically, Table 1 and Table 2 exhibit the data from the Protein Blast Alignment Tool data from the calcium binding fusion domains, labeled FL1 and FL2 respectively, that compare the spike proteins of COVID-19 (SARS-CoV-2) with SARS-COV and Rubella utilizing cited reference data; GenBank: QHD43416.1 (CoV-2), NCBI Reference Sequence: NP_828851.1(CoV-1), GenBank: ACN50046.1. (Rubella), GenBank: NP_828851(Human coronavirus229E) and GenBank: AD177360.1 (hemagglutinin [Influenza A virus (A/Boston/136/2009(H1N1))]).
The results demonstrated a 100% and a 95% correspondence respectively between the postulated FL1 and FL2 domains in SARS-CoV-2 (COVID-19) compared to the known FL regions for SARS-CoV-1 described in 2017. Additionally, the less pathogenic Alpha coronavirus 229E (HCoV-229E) has a solitary FL2 domain and a reduced homology length compared to the SARS-CoV-2 (COVID-19) FL2. Similarly, amino acids 49 to 55 of the Rubella Virus membrane glycoprotein E2 virus also have a smaller, but significant homology with the FL2 domain. In contrast, the Influenza H1N1 hemagglutinin (HA) protein has no significant similarity to any of the CoV FL domains. The reduced homology of HCoV-229E’s single calcium-binding domain to SARS-CoV-2 suggests attenuation of HCoV-229E, which is consistent with HC0V-229E having crossed species barriers to infect humans decades or centuries ago.

采用 NCBI pBlast 工具对假设进行检验，即 SARS-CoV-2 是否含有类似近期在风疹和 SARS-CoV-1 中发现的钙依赖性融合结构域。通过美国国家生物技术信息中心（National Center for Biotechnology Information）的蛋白质 Blast 程序，对代表融合环 1（FL1 = 氨基酸 798-819）和融合环 2（FL2 = 氨基酸 835-855）的 S 蛋白中两个相关的氨基酸区域进行了氨基酸比较。具体而言，表 1 和表 2 展示了蛋白质 Blast 对齐工具中钙结合融合结构域（分别标记为 FL1 和 FL2）的数据，这些数据通过引用数据将 COVID-19（SARS-CoV-2）的刺突蛋白与 SARS-CoV 和风疹进行比较；GenBank：QHD43416.1（CoV-2），NCBI 参考序列：NP_828851.1（CoV-1），GenBank：ACN50046.1（风疹），GenBank：NP_828851（人类冠状病毒 229E）和 GenBank：AD177360.1（血凝素 [流感 A 病毒（A/Boston/136/2009(H1N1))])。结果显示，与 2017 年描述的 SARS-CoV-1 的已知 FL 区域相比，SARS-CoV-2（COVID-19）中假设的 FL1 和 FL2 结构域分别表现出 100% 和 95% 的一致性。此外，与 SARS-CoV-2（COVID-19）的 FL2 相比，致病性较低的 Alpha 冠状病毒 229E（HCoV-229E）具有单个 FL2 结构域，且同源性长度有所减少。同样，风疹病毒膜糖蛋白 E2 的氨基酸 49 至 55 也有与 FL2 结构域较小但显著的同源性。相反，流感 H1N1 血凝素（HA）蛋白与任何 CoV FL 结构域均无显著相似性。HCoV-229E 的单个钙结合结构域与 SARS-CoV-2 的同源性降低表明了 HCoV-229E 的减弱，这与 HCoV-229E 在数十年或数百年前跨越物种屏障感染人类的事实相一致。