Mass Cytometry (CyTOF) FCS files from Priest et al. 2024. Human PBMC from longitudinal analysis of COVID-19, Bacterial Sepsis, mRNA vaccination cohorts.

Mass Cytometry (CyTOF) FCS files from Priest et al. "Non-classical CD45RBlo memory B-cells are the majority of circulating antigen-specific B-cells following mRNA vaccination and COVID-19 infection." Research Square 2024. 

Files are already normalised, debarcoded, gated, batch corrected and compensated as described in Priest et al. 

Data is from Human PBMCs of londitudanal cohorts of Severe COVID-19, Sepsis and mRNA vaccine recipients. 

Samples were barcoded, mixed and then split magnetically before staining with seperate antibody panels for CD3+ (CD4, Treg, Tfh, CD8, gdT) or CD3- (B cells, DC, NK, Monocytes) to give approximatly 1280 FCS files from 218 individuals. 

A follow up experiment with a B-cell specific panel and Tetramers is included. 

Patient level metadata and antibody panel details are included.