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Effect of miR17-92 and miR-106b-25 loss in primary calvarial cells

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https://www.ncbi.nlm.nih.gov/sra/SRP146250
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资源简介:
Gene expression profiling of primary calvarial cells with miR-17-92:miR-106b-25 conditional deletion Overall design: 10-day-old primary calvarial cells of floxed miR-17-92(fl/fl):miR-106b-25(-/-) mice were isolated with collagenase digestion, cultured in alpha-MEM containing 10% fetal bovine serum, and infected with adenoviruses expressing Cre or YFP. 3 days after adenovirus infection, RNA was extracted with Trizol, purified with Zymo spin columns, and subjected to RNA-seq
创建时间:
2019-07-07
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