RNAseq of two recombinant CHO cell lines differing only in the factor “produced monoclonal antibody\"

Major improvements have been made in the production of recombinant proteins with the adaptation of CHO cells to growth in suspension and the development of rich and balanced media which can be applied in batch processes or translated to perfusion. In this study we evaluated the difference of the mRNA profile of two recombinant cell lines differing only in the factor “produced monoclonal antibody”, more precisely, the expressed mAbs differ in their variable regions resulting in varying expression profiles. The cell lines were cultivated in a semi-continuous process mode at approx. 40 × 106c/mL with a viability of higher than 90% for several days. This strategy was chosen to prevent effects describing the adaptation of cells to classical growth characteristics such as exponential and stationary culturing phases of non-continuous processes.