Genome-wide expression analysis of Arabidopsis Col-0 plants under BTH treatment and high or low humidity

The goal of this study is to demonstrate the global expression profile of four-week-old Arabidopsis Col-0 wild type plant leaves in response to high or low air humidity, under water or BTH (SA analog) treatment. Overall design: Col-0 plants were treated with high or low humidity for 24 h and then sprayed with 100 µM BTH. Leaves were collected at 0 and 12 h after BTH treatment. Total RNA was extracted from the tissues using Trizol Reagent (Invitrogen) based on the manufacture's instruction, and genomic DNA was removed using the DNaseI (Invitrogen). RNA was further purified by using the RNeasy MinElute Cleanup kit (QIAGEN). RNA quality check, library construction, and RNA sequencing were performed by the Majorbio company.