CIL:9529, notopthalmus viridescens, erythrocyte. In Cell Image Library

Newt (Notopthalmus viridescens) erythrocytes were isolated in 0.1M KCl, spread on the surface of 5mM Na-citrate, picked up on formvar-carbon films, fixed with glutaraldehyde and paraformaldehyde and negatively stained with sodium tetraborate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 55 degrees. A grouped image of the same field at a 45 degree tilt provides an oblique stereo view of the tangle of chromatin fibers emanating from the dispersed nucleus.