Alingment of COI sequences of Ch. plumosus group species and Tables 1-5.

The alignment that was used for analysis for paper "Karyotype characteristics and gene COI sequences of Chironomus bonus Shilova & Dzhvarsheishvili, 1974 (Diptera, Chironomidae) from the South Caucasus". The alignment consists of DNA data (COI gene sequences) from both GenBank and BOLD (Ratnasingham and Hebert 2007) databases for the species Chironomus dorsalis Meigen, 1818 (JN887047.1); Ch. balatonicus (JN016826.1); Ch. muratensis (AF192194.1); Chironomus sp. prope agilis (AF192190.1); Ch. borokensis (AB740261.1); Ch. usenicus (JN016807.1, JN016809.1, JN016808.1); Ch. entis (KF278213.1, KF278212.1, KJ085531.1, KJ087284.1, KJ089893.1, GBDPC429-14, MGOCF102-16); Ch. plumosus (AB740263.1, AB740262.1, JN016830.1, JN016829.1, CHBAL014-20, CHIFI298-16, CHIFI299-16, LEFIJ3947-16, LEFIJ3948-16, PGBAL006-19, PGBAL007-19, PGBAL009-19, PGCBG089-20, BSCHI661-17, BSCHI063-11, BSCHI115-17, BSCHI219-17, BSCHI284-17, BSCHI350-17, BSCHI517-17, BSCHI644-17, GBDP44143-19, GBDP44180-19, LC050899.1, LC050900.1, JCDB364-15, JCDB363-15, GBDP11685-12, GBDP11686-12, GBDP11687-12, GBDP12282-12, XJDQD1039-18, XJDQD1037-18, XJDQD1038-18, XJDQD1036-18, MN750315.1, GBDPC430-14, SDP408034-15, GBDPC133-14, GBDPC138-14, GBDPC144-14, GBDPC166-14); and Pagastiella orophila (Edwards, 1929) (JN265047.1).Table 1. Estimates of evolutionary divergence between sequences of true Ch. plumosus cluster. The number of base substitutions per site (%) from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model (Kimura 1980). Table 2. Estimates of evolutionary divergence between sequences of Ch. entis cluster. The number of base substitutions per site (%) from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model (Kimura 1980). Table 3. Estimates of evolutionary divergence between sequences of Ch. borokensis cluster. The number of base substitutions per site (%) from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model (Kimura 1980). Table 4. Estimates of evolutionary divergence between sequences of Ch. plumosus from Finland and sequences of Ch. balatonicus, Ch. muratensis and Ch. agilis 2. The number of base substitutions per site (%) from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model (Kimura 1980).Table 5. Estimations of the age of the most recent common ancestors (TMRCAs) for DNA clades.