Real-time quantitative PCR analysis of human colonic HT-29 cells under insulin stimulation
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE51677
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资源简介：
Compared mRNA expression between control and insulin treated cells. HT29 cells were treated with 100nM Insulin for 24h. The RT2 Profiler PCR Arrays PAHS-30C (SA Biosciences, MD, USA) was designed to analyze 84 genes related to human insulin signaling pathway. The RT-PCR was carried out using an ABI PRISM® 7000 Sequence Detection System (Applied Biosystems, Foster City, CA).The expression of HSD11B2 for the 3 experiments concerned was monitored in parallel by real time PCR which confirming significant downregulation by insulin. qPCR gene expression profiling. RNA from 3 separated experiments was collected.
创建时间：
2014-09-01
