Specific pathways are selectively enriched between WT and Mist1−/− acinar cells.

Ingenuity Pathway analysis (IPA) was performed for genes exhibiting preferential H3K4Me3 enrichment in either genotype. A right tailed Fisher's exact test was used to prioritize the most significant biological functions based on −log of p-value. The ratio was calculated by taking the number of genes in a given pathway that meet the cutoff criteria, and dividing this total by the number of genes that make up the pathway. The orange line represents the threshold where pathways are considered over-represented. The ten most represented molecular and cell _erulean_g pathways are shown for gene sets that are preferentially enriched for H3K4Me3 in (A) wild type and (B) Mist1−/− samples. (C) The top eight disease-related pathways represented by enriched genes within Mist1−/− samples are shown along with inflammatory disease and response, due to the relationship of MIST1 with pancreatitis. (D) Identical IPA performed for these disease related pathways using the list of genes that are differentially enriched and differentially expressed between WT and Mist1−/− tissue.