Identifying super-enhancer-derived signatures with prognostic value through selective inhibitors (Microarray gene expression data)

CDK12 and CDK13 promote transcription elongation within the gene body and regulate the processivity of RNAPII. THZ531 inhibits the enzymatic activity of CDK12 and 13 through covalent binding. Gene expression profiling was performed to investigate the THZ531-induced transcription effect, and search the subset of sensitive genes in osteosarcoma cell lines, U2-OS and SJSA-1. Total RNA was extracted from U2-OS or SJSA-1 cells treated with DMSO (control), 50, 200, and 800 nM THZ531 for 6 hours, respectively, using TRIzol reagent.An Affymetrix GeneChip® PrimeView™ Human Gene Expression Array was used for the microarray analysis. The hybridization data were analyzed using Affymetrix GeneChip Command Console Software.