Transcriptome sequencing of Wild-type and psmb11-/- thymic epithelial cells. Mus musculus

Purpose: as regulator of protein degradation, proteasomesregulate pratically all cellular functions. We assume that psmb11 specific expression by cortical thymic epithelial cells (cTECs) could have far reaching effect on cTECs biology and their involvment in thymocytes' education. Method: We analyzed the poly(A)+ transcriptome of TEC populations by RNA-sequencing (RNA-Seq) in order to gain novel insights into psbm11 functions Results: We demonstrate here that psmb11-deficiency affects the transcription of 450 genes in cortical thymic epithelial cells Conclusion: Our study reveals that one of psmb11 cellular roles could rely on transcriptional regulation and more important, inhibition of mTEC-lineage engagement in cTEC differenciation. Overall design: The mRNA profiles of cTEC, mTEClo and mTEChi from 11 thymi of 6-8 weeks-old wilt type (WT) and psmb11-/- mice were generated by RNA-sequencing using Illumina HiSeq2000