Identification of genes regulated by Fer2 in PAM neurons using an overexpression approach. Identification of genes regulated by Fer2 in PAM neurons using an overexpression approach

The transcription factor Fer2 plays a neuroprotective role in the dopaminergic neurons of the fly brain, but the underlying molecular mechanisms are not understood. By performing RNA-seq on isolated dopaminergic PAM neurons after Fer2 constitutive overexpression, we identified over one hundred genes differentially regulated in response to PAM-specific Fer2 overexpression. Overall design: RNA-seq on isolated dopaminergic PAM neurons from 14 day-old flies overexpressing a UAS-Fer2::FLAG transgene driven by a PAM specific GAL4 (R58-GAL4). Flies not carrying the UAS-Fer2::FLAG transgene were used as controls. N=3 per group.