Evolution and recovery of the gut microbiota during and after rifampicin treatment in Blattella germanica

The German cockroach Blattella germanica is an omnivorous insect .We have developed an experimental design involving one control population and two treated populations during 10 days with 0.02% rifampicin, one of which was supplemented with faeces from the beginning. First, we dissected the hindgut of females every 2 days. Then, rifampicin was removed and the experiment continued to check the recovery of the gut microbiota without antibiotic. The rifampicin-only treated population was divided in two sub-populations, one of which was now supplemented with faeces. We dissected the hindguts of cockroach every 10 days until 60.