Transcriptional profiling of striatum from a mouse model of Huntington's disease (Q140) crossed with Msh3 heterozygous and homozygous mice [Msh3_striatum]

As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntingtons disease (HD) through various computational modeling efforts. Striatum was isolated from 12-month-old Msh3+/- mice and Msh3-/- (KO) crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Msh3+/-, Msh3-/-, Q140, Q140 X Msh3+/- and Q140 X Msh3-/-, 8 replicates per genotype. RNASeq analysis was performed on samples from the striatum tissues of 12-month-old Msh3+/-, Msh3-/-, Q140, Q140 X Msh3+/- and Q140 X Msh3-/-, and wild-type mice. N = 8 per group (4F/4M).本数据集为加州大学洛杉矶分校（UCLA）与CHDI合作项目的一部分，UCLA将通过多项计算建模研究发现的、与亨廷顿舞蹈症（Huntingtons disease, HD）相关的123个基因构建敲除（knockouts, KOs）模型。研究人员从12月龄的Msh3+/-小鼠、与Q140敲入（knock-in, KI）HD小鼠杂交得到的Msh3-/-（KO）小鼠及其对应对照中分离纹状体组织。本次实验对6种基因型的样本开展转录组测序（RNASeq）分析，涉及的基因型分别为野生型（WT）、Msh3+/-、Msh3-/-、Q140、Q140 × Msh3+/-及Q140 × Msh3-/-，每组设置8个生物学重复。所有转录组测序样本均取自12月龄上述基因型小鼠的纹状体组织，每组样本量N=8，其中雌性、雄性各4只。