Sequential regulation of glutamatergic and subtype-specific projection neuron identities in the developing cerebral cortex

Purpose:To asses changes in gene expression profiles of cortices from the P0 wild type littermate controls and Tle4-/- null mutant mice. Methords:Total RNA was isolated and sequenced using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered significantly changed whicn perform P value <= 0.05 Results: Compared to controls, 306 genes were significantly down-regulated in the Tle4 null mutant mice, and 338 genes were significantly up-regulated in the Tle4 null mutant mice. Cortex RNA profiles of P0 Wild type mice brain, and Tle4-/- null mutant mice brain研究目的：评估P0野生型同窝对照小鼠与Tle4-/-纯合敲除突变小鼠大脑皮层的基因表达谱变化。 实验方法：提取总RNA后使用Illumina High-seq 2500平台进行测序；采用TopHat软件对原始测序数据进行分析；当P值≤0.05时，判定对应基因的表达存在显著差异。 实验结果：与对照组相比，Tle4-/-纯合敲除突变小鼠脑中共有306个基因显著下调，338个基因显著上调。 数据集样本：P0野生型小鼠及Tle4-/-纯合突变小鼠的大脑皮层RNA表达谱。