RNA-seq of YAP's role in hDPCs

We report the application of RNA sequencing technology for high-throughput profiling of gene expression in YAP knockdown (siYAP) adult human dental pulp stromal cells (hDPCs).To knockdown YAP in hDPCs we transfected cells with YAP specific siRNAs and the scrambled RNAs. Cells were derived from a 18~22-year-old healthy male human donor. To induce pulp inflammation we used LPS to treat cells. Total mRNA was collected using TRIzol ® (Invitrogen, CA, USA) solution. Then all the extraction procedures were conducted according to manufacture’s instructions for RNA extraction from homogenized tissues. After quality checks they were subjected to the high-throughput RNA-sequencing. Every group consists of 2 replicates which were from 2 independent experimental repeats. Examination of RNA expression in hDPCs after YAP knockdown or LPS treatment respectively.