Comprehensive Analysis of SARS-CoV-2 Spike Gene: Mutation Variability, GC/AT Content, and Evolutionary Dynamics Across Randomly Selected 186,457 Genomes

This dataset provides a detailed genomic analysis of the SARS-CoV-2 Spike (S) gene, based on 186,457 high-quality, full-length genomes aligned to the Wuhan-Hu-1 reference (NC_045512.2). Key findings include: Mutation Variability: Mutation frequencies were calculated at both nucleotide and amino acid resolutions, identifying critical hotspots in regions such as the Receptor-Binding Domain (RBD), N-terminal Domain (NTD), and Furin cleavage site. Results highlight strong selective pressures linked to immune escape and transmissibility, offering a dynamic view of viral adaptation over time. GC/AT Content: Total sequences analyzed: 186,457 Total bases: 712,638,654 Overall GC%: 36.13% Overall AT%: 60.88% Detailed breakdown: Total G: 127,357,894 Total C: 130,107,079 Total A: 204,006,970 Total T: 229,838,111 Evolutionary Dynamics: The dataset enables tracking of evolutionary trends, prioritization of surveillance targets, and supports vaccine and therapeutic development.