Genome-wilde expression profiling analysis of yeast cells lacking the decapping activator Scd6

To assess the role of the decapping activator Scd6 in mRNA decay, we used RNA-Seq to analyze the expression profile of yeast cells harboring a deletion of the SCD6 gene. Consistent with our recent model for decapping regulation, we found that Scd6 targets a small number of specific mRNAs in yeast cells. Interestingly, degradation of Scd6-targeted transcripts also requires the functions of the decapping activators Pat1, Lsm1, and Dhh1, suggesting that Scd6 functions together with Pat1, Lsm1, and Dhh1 in promoting mRNA decapping.为评估脱帽激活因子Scd6在mRNA降解中的作用，我们采用RNA测序（RNA-Seq）分析了携带SCD6基因缺失的酵母细胞的表达谱。与我们近期提出的脱帽调控模型一致，我们发现Scd6在酵母细胞中靶向少量特定mRNA。有趣的是，Scd6靶向转录本的降解同时依赖于脱帽激活因子Pat1、Lsm1及Dhh1的功能，这表明Scd6可与Pat1、Lsm1及Dhh1协同发挥促进mRNA脱帽的功能。