Expression profiling of lung cancer cells reconstituted with SMARCA4 WT and mutant K785R after SMARCA2 knockdown. Expression profiling of lung cancer cells reconstituted with SMARCA4 WT and mutant K785R after SMARCA2 knockdown

Using RNA-seq, we identified the expression changes in lung cancer cells (NCI-H1944) transduced with control or SMARCA2-targeting hairpins. We also identified the SMARCA2 program that is rescued by SMARCA4 WT and SMARCA4 K785R transduced. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. The ID of this project in Genentech's ExpressionPlot database is PRJ0034746 Overall design: NCI-H1944 cells were lentivirally transduced with LACZ, SMARCA4 WT and SMARCA4 K785R and selected with blasticidin for 5 d. Cells were then transduced with nontargeting or SMARCA2-targeting hairpins in triplicate. After 72 h puromycin selection, RNA was isolated from cell pellets.