MHC-dependent activation of T_EFF cells by APCs.

(A) Mice received naïve gBT-I or gDT-II cells prior to HSV-1 skin infection and DNFB treatment on opposite flanks. Analysis of IFN-γ+ gBT-I and gDT-II cells from skin (collagenase digestion) 5 days post-infection. Data representative of n = 2 experiments with skin pooled from 4 mice each. (B–D) Wild-type (WT) and H-2Kb−/− mice or WT→WT and H-2Kb−/−→WT bone marrow chimeric mice were subjected to HSV-1 skin infection and transferred with in vitro activated gBT-I cells 3 days post-infection. (E) WT→WT and WT→H-2Kb−/− mice received naïve gBT-I cells prior to infection. (B–E) Analysis of IFN-γ+ gBT-I cells from axillary LN (B) and epidermis (Epi, dispase digestion, C–E) 5 days post-infection. **, P<0.01; ***, P<0.001; ns, not significant by Mann Whitney test; n = 7–14 mice/group from 3–5 experiments. (F,G) WT and I-A/E−/− mice were subjected to HSV-1 skin infection and transferred with in vitro activated gDT-II cells 3 days later. Analysis of IFN-γ+ gDT-II cells from skin (collagenase digestion) and axillary LNs 5 days post-infection. *, P<0.05; **, P<0.01 by Mann Whitney test; 3 experiments with n = 2–4 mice/group; symbols for skin represent values from pooled tissues. (H) WT→WT and WT→I-A/E−/− mice received naïve gDT-II cells prior to infection. Analysis of IFN-γ+ gDT-II cells in skin (collagenase digestion) and axillary LNs 5 days post-infection. *, P<0.05 by Mann Whitney test; n = 9–19 mice/group from 3 experiments.