Consequences of a loss of Ash2l in the bone marrow.. Consequences of a loss of Ash2l in the bone marrow.

The Ash2l protein is a member of KMT2 enzyme complexes, which catalyse the (tri-)methylation of lysine 4 of Histone H3. H3K4me3 is considered a marker of actively transcribed genes. We determined changes in gene expression as a consequence of a conditional loss of Ash2l in the bone marrow. The expression data from the bone marrows of mice with floxed Ash2l exon 4 alleles without (control) or with an Mx1-Cre transgene (KO) and treated with synthetic dsRNA to induce recombination and the loss of Ash2l protein expression were compared. Overall design: The expression data from LSK cells of the bone marrow from 3 control and 3 Ash2l KO mice were analysed and the fold changes calculated.