SMG1 effects on α-synuclein expression are independent of its function in the mRNA surveillance pathway.

Silencing the UPF1 protein, a key regulator of mRNA surveillance, has no effect on either p-syn or t-syn levels, indicating that disruption of the mRNA surveillance pathway is not the mechanism through which SMG1 affects α-synuclein. The antibodies are the same as in Figure 3, with the addition of anti-UPF1 antibody (α-UPF1). siRNAs used are indicated across the top of the westerns. Results are representative of at least 3 independent replicates.