renal ischemia vs sham - 7d. Rattus norvegicus

this experiment was designed to determine the effect of ischemia reperfusion on angiogenesis gene expression after 7 days of recovery Keywords: disease state Overall design: rat angiongenesis gene chips were designed. RNA was compared from kidneys of post-ischemic rats and sham-operated rats. 4 pairs of rats/ 1 hybridization each. data were first analyzed to combine replicate spot intensities for each hybridization; these normalized ratios were then averaged across each hybridization to determine alteration in gene expression in response to ischemia reperfusion.