Effects of inbreeding and elevated rearing temperatures on strategic sperm investment

Males often strategically adjust the number of available sperm based on the social context (i.e., sperm priming response), but it remains unclear how environmental and genetic factors shape this adjustment. In freshwater ecosystems, high ambient temperatures often lead to isolated pools of hotter water in which inbreeding occurs. Higher water temperatures and inbreeding can impair fish development, potentially disrupting sperm production. We used guppies (Poecilia reticulata) to investigate how developmental temperature (26 °C, 30 °C) and male inbreeding status (inbred, outbred) influence their sperm priming response. We also tested if sperm priming was affected by whether the female was a relative (sister), and whether she was inbred or outbred.
We ran two separate analyses to address our research questions. First, we ran a linear mixed model (LMM) to investigate the effects of male inbreeding status (inbred, outbred), rearing temperature (warm, control), and social environment (no fem..., , , # Effects of inbreeding and elevated rearing temperatures on strategic sperm investment

[https://doi.org/10.5061/dryad.wstqjq2vt](https://doi.org/10.5061/dryad.wstqjq2vt)

## Description of the data and file structure

**-- Variables description for csv. files --**

---

**For all files (\"Analysis_in_method\", \"Analysis_1\", \"Analysis_2\"):**

Male_id: male identity

Adult_age: male's age at testing (days)

Male_size: male's body size at testing (mm)

Brood_id: male family identity

Male_breeding_status: male inbreeding status (\"Inbreed\" or \"Outbreed\")

Male_temp: male rearing temperature before maturation (\"Control\" = 26°C; \"Warm\" = 30°C)

sperm_count: male sperm count. The cell was left empty if no sperm was stripped from the male.

---

**For both \"Analysis_in_method\" and \"Analysis_1\":**

Social_environ: male social environment (\"No_female\" = no female; \"Nonrelated_outbred\" = a non-related outbred female; \"Related\" = a related female)

---

**For both \"Analysis_1\" and \"Analysis_2\":**

...