System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5âUTR G4 structure to promote Gnai2 RNA translation [Polysome profiling]
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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资源简介：
In this study, we deciphered the functions of Dhx36 and related molecular mechanisms in muscle stem cells and muscle regeneration. We found Dhx36 was highly induced in activated muscle stem cells during regeneration induced by injury. Conditional inactivation of Dhx36 in mouse muscle stem cells caused significant impaired regeneration, which was due to the dramatically decreased cell proliferation. Dhx36 was a well-known RNA helicase for binding/unwinding DNA/RNA G-quadruplexes and it was dominantly expressed in cytoplasm of proliferating myoblast. To examine whether Dhx36 regulates translation process, we conducted polysome profiling followed by RNA-seq in WT and Dhx36 KO myoblast cells.
提供机构：
The Chinese University of Hong Kong
创建时间：
2022-02-20
