Cat mtDNA fragments

Nine ND5 gene fragments were amplified via multiplex PCR and either sequenced by NGS in bulk following the “aMPlex Torrent” workflow, or reamplified individually by nested PCR and sequenced by pyrosequencing (see Ottoni et al, 2017, Nature Ecol Evol in press). The nine sequences obtained by “aMPlex Torrent” for each specimen were concatenated using a 10 N intervening spacer. For the specimen sequences that were obtained only by pyrosequencing, the N spacer was extended to allow alignment of all sequences. The coordinates of the fragments, excluding primers, numbered according to the Felis silvestris mtDNA reference sequence (NC001700) are: Frag_7: 12918-12961; Frag_2: 13052-13102; Frag_8: 13263-13333; Frag_3: 13513-13569; Frag_4: 13746-13807; Frag_1: 13945-13980; Frag_5: 14004-14012; Frag_9: 14170-14228; Frag_6: 15071-15131.