Arbuscular mycorrhizal symbiosis under short- and long-term drought in Lotus japonicus

[ENG:]The dataset contains results from experiments investigating the impact of arbuscular mycorrhizal fungi (Rhizophagus irregularis) on the responses of Lotus japonicus under full irrigation and during short-term (1 week) and long-term (3 weeks) drought. The data include morphological measurements such as shoot and root length, leaf number, and fresh and dry biomass, as well as root architecture parameters including total root length, volume, diameter, and number of tips. Physiological indicators such as leaf relative water content (RWC) and proline concentration are also included, along with chlorophyll fluorescence parameters such as Fv/Fm, Y, ETR, and NPQ. Additionally, the dataset provides information on the level of root colonization by AMF assessed using microscopy (gridline-intersect method) and quantified by qPCR. The dataset covers six experimental treatments: plants inoculated with mycorrhizal fungi and control plants supplemented with two phosphorus concentrations, under drought and full irrigation. Experiments were conducted in a phytotron at the Poznań University of Life Sciences, in the Biocentrum building at Dojazd 11, Poland. Methods included root scanning and image analysis (WinRHIZO), chlorophyll fluorescence measurements using an OS5p fluorometer, leaf water status assessment by the Weatherley method (1950), proline determination following Bates et al. (1973), and AMF colonization evaluation by microscopy and qPCR using species-specific gene markers.For more information, please see the ReadMe file.