Effects of running for 1h on gene expression in Achilles tendon cells of SD rats

The Achilles tendon is the thickest tendon in the human body, and Achilles tendinopathy is its most prevalent disorder, often considered a consequence of overuse. While disruption to the collagen fibers represents a significant manifestation of Achilles tendinopathy, strikingly little is known about the mechanisms by which healthy tendon accumulates damage in vivo.As existing studies of tendon biomechanics and mechanobiology predominantly relied on in vitro or ex vivo experiments on isolated tissues, it is still largely unknown whether and how disruptions occur to the collagen molecules in healthy Achilles tendons following physiological activities. We reported the first RNA-seq analysis reflecting transcriptome changes in healthy rat Achilles tendons following running, providing a resource for future investigations in tendon mechanobiology and sports medicine. Overall design: Rats in the running group (0 h p.r. group) were sacrificed immediately after 1 h of treadmill running. Fresh Achilles tendon tissues from the right legs of the running and naïve control (NC group) rats were immediately dissected and placed in RNALater™ to prevent RNA degradation. The total RNA was extracted after the tissues were mechanically pulverized in TRIzol. After verification of the RNA integrity by Qsep100 (RNA integrity number > 7.0), polyA mRNAs was purified from total RNA using mRNA capture beads. Then the mRNA was reverse-transcribed to create a cDNA library. VAHTS Stranded mRNA-seq Library Prep Kit for Illumina V2 was used for library preparation. RNA sequencing was performed using the Illumina NovaSeq 6000 sequencer. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different tissues at two groups.Comparative gene expression profiling analysis of RNA-sea data for 0 h p.r. group and NC group.