RNA-seq analysis with WT and Ninj2-deficient mouse oligodendrocytes.

Loss of Ninj2 in oligodendrocytes reduced the population of Olig2+ oligodendrocytes. In order to elucidate the molecular basis underlying the dysmyelination induced by Ninj2 deletion, we performed RNA-Seq analysis with WT or Ninj2-deficient mouse oligodendrocytes to identify specific differentially expressed genes. Overall design: RNA-Seq analysis with WT or Ninj2-deficient mouse oligdendrocytes were performed. All RNA-Seq data were aligned to mm10 by Hisat2 v2.0.5. FeatureCounts v1.5.0-p3 and StringTie(v1.3.3b) were used to generate gene counts. Differential expressed genes were identified using DESeq2 with fold change>2.0 and p<0.05.