Yeast solutions and hyperpolarization enable real-time observation of metabolized substrates even at natural abundance

This data corresponds to the following paper:

Title: Yeast solutions and hyperpolarization enable real-time observation of metabolized substrates even at natural abundance

Journal: Analytical Chemistry

Authors: Josh P. Peters, Charbel Assaf, Farhad Haj Mohamad, Eric Beitz, Sanjay Tiwari, Konrad Aden, Jan-Bernd Hövener and Andrey N. Pravdivtsev

The data is organized with respect to the subfigures in figure 2 to 6.

A description of acquisition parameters is provided in the "Acquisition parameters.xlsx" file for each dataset.

Figure 2:

- Overview of pyruvate metabolism in yeast cells using hyperpolarized and 13C labeled [1-13C]pyruvate. 

Hyperpolarized and thermally polarized spectra, if applicable, are included. 

Figure 3:

- Overview of pyruvate metabolism in yeast cells using co-hyperpolarized [1-13C] and [2-13C]pyruvate at an n.a. of 13C. 

Hyperpolarized and thermally polarized spectra, if applicable, are included.

Figure 4:

- Overview of fumarate metabolism in yeast cells using hyperpolarized and 13C labeled [1,4-13C2]fumarate

Hyperpolarized and thermally polarized spectra, if applicable, are included.

Figure 5:

- The fitted conversion exchange rate constants as a function of the yeast concentration. 

Hyperpolarized and thermally polarized spectra, if applicable, are included.

Figure 6:

- Metabolic data from pyruvate metabolism in yeast depending on the position of the yeast in the NMR tube. We 

Hyperpolarized and thermally polarized spectra, if applicable, are included.