Characterization of gene profile between ALDH high LNCaP cells and ALDH low LNCaP cells

Acquisition of prostate cancer stem cells (PCSCs) manifested during androgen ablation therapy (ABT) contributes to castration-resistant prostate cancer (CRPC). However, little is known about the specific metabolites critically orchestrating this process. Here, we show t+B13hat IMPA1-derived inositol enriched in PCSCs is a key metabolite crucially maintaining PCSCs for CRPC progression and ABT resistance. Overall design: For fluorescence-activated cell sorting, cells were labeled with ALDEFLUOR Kit and sorted using MoFlo Astrios Cell Sorter (BECKMAN COULTER) in two ways including highest 10 % ALDH positive cells (ALDHhigh) and the lowest 10% ALDH negative cells (ALDHlow/-), following by cell culture and immunoblotting.