Gradients3-KM1906-PCPN-UW

PCPN Samples were collected on the SCOPE Gradients 3 cruise in the north Pacific Ocean. The carbon and nitrogen content of particulate material (PC, PN) was collected by semi-automated and sequential filtration of the ship’s underway flow through seawater onto a combusted GFF filter. Select samples had a piggybacked GFF filter holder in sequence for collection of dissolved organic material and/or evaluation of trapping efficiency of GFF. Following filtration, filters were folded in half and placed in combusted aluminum foil and stored at -80°C for later analysis. Post-cruise, all filters were thawed, acidified, and dried overnight at 60°C. Samples were then balled into Sn boats and plated for analysis. Carbon and nitrogen content was analyzed at University of Hawaii at Manoa's S-Lab (SOEST Laboratory for Analytical Biogeochemistry) (http://www.soest.hawaii.edu/S-LAB/equipment/slab_CNS.htm). Depth of 6m refers to the depth of the seawater intake on the R/V Kilo Moana (KM). The ship’s location, sea surface temperature and salinity from ship’s underway instrumentation are matched to the mid-point of each PCPN filtration time.