Transcriptome analysis of wild-type and VaAQUILO overexpressing Arabidopsis

To gain the possible direct or indirect targets of VaAQUILO, RNA-Seq was carried out on three biological replicates of wild-type and 3 transgenic Arabidopsis lines mixture (#1, #2 and #3) under normal and cold treatment conditions (Based on the distribution of samples in a first PCA plot, the two most similar replicates per condition were considered for further statistical analyses) . Examination of gene expression changes after overexpression of VaAQUILO in Arabidopsis and identification of VaAQUILO downstream genes.