S1 Table -

Comparison of NGS-PrimerPlex and other tools functionality: a–choosing genome regions to amplify by gene names and their parts. b–primer design for all chosen genome regions. c–checking primers for non-target hybridization and non-target amplification. d–checking primers for covering variable genome sites that contain high-frequent SNPs. e–automatic primer distribution for several multiplex reactions, considering secondary structures and non-target amplicons that can be formed by primers from different pairs. f–primer design for nested PCR. g–primer design for anchored PCR when the target region is amplified from one sequence-specific primer and one primer complemented to the adapter sequence. It can be useful for the detection of gene fusions. h–has graphical or web-interface yes/no–means that this tool includes or does not include such functionality, respectively. ± –means that such functionality is partial (e.g. distribution among pools is restricted by a maximum of 15 primer pairs in one pool)? –means that such functionality was claimed but the tool wasn’t acceptable (XLSX)