ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes

ETV1 (ER81) was identified as a highly enriched transcription factor in rapidly conducting atrial and His-Purkinje cardiomyocytes. Previous work has shown that ETV1 is a critical factor in determining fast conduction physiology in the heart. This high-throughput sequencing project aims to uncover ETV1-dependent gene networks in cardiomyocytes. These results provide insight into the role of ETV1 in the establishment of rapid conduction zones in the heart. Overall design: Neonatal rat ventricular myocytes were isolated and purified from postnatal day 1 Sprague-Dawley wild type male and female rats using Miltenyi Biotec rat neonatal cardiomyocyte isolation kit (Miltenyi Biotec, 130-105-420) according to the manufacture's protocol. The final myocyte cultures contained enriched cardiomyocytes at a density of 3X?10^6 cells per 35-mm dish. Myocytes cultures were transduced with either Ad-ETV1-GFP or Ad-GFP 24 hours after plating. RNA was extracted at day 4 post-transduction to analyze transcriptional differences with the overexpression of ETV1 in cardiomyocytes.