Transcriptome modulation by loss-of-YAP1/TAZ in SK-N-MC Ewing Sarcoma cells. Transcriptome modulation by loss-of-YAP1/TAZ in SK-N-MC Ewing Sarcoma cells

YAP1 (Yes-associated protein 1) and TAZ (transcriptional coactivator with PDZ-binding motif, or WWTR1) are nucleo-cytoplasmic shuttling proteins that can function in the nucleus as transcriptional coactivators. Here we sought to evaluate which genes are regulated by endogenous levels of YAP/TAZ. We compared SK-N-MC cells transfected with a control non-targeting siRNA with cells transfected with a mix of siRNA targeting both YAP and TAZ. Overall design: SK-N-MC cells growing at 70-80% of confluency in standard growth conditions were transfected with the indicated siRNAs. The following day, growth medium was renewed and cells were incubated for 48 more hours, and then harvested for total RNA extraction. 4 independent biological replicates were plated, transfected and harvested in parallel for each siRNA transfection.