Metagenomic analysis in a mouse model of steatohepatitis

It is previously established that the ratio of Bacteroidetes and Firmicutes, the two dominant phyla that make up over 90% of bacterial phylogenetic types in the human distal gut, is modulated by the diet; in particular a diet enriched in fat causes a modulation of the intestinal microbiota composition that results in a decreased proportion of Bacteroidetes which is compensated by a robust increase in the proportion of Firmicutes. The goals of this study are to confirme these findings analyzing the composition of microbiota in mice fed a High Fat diet and fructose (HFD-F), and to evaluate the effects of two bile acid receptors ligands, Ursodeoxycholic acid (UDCA) and Obeticholic acid (OCA), on intestinal flora in this mouse model of NASH. Here we report the information obtained from metagenomics workflow for each sample.